Catalysis encapsulated in micro- and nanostructures

Project I Bioreactors: Bioreactor boundaries and the influence on encapsulated reactions

Two possible enzymatic systems are considered Terminal deoxynucleotidyl transferase or T7 RNA polymerase.

  • Interactions between metal-ions and PC membranes: How is the availability of metal ion co-factors influenced by the membranes?
  • Interactions between components composing the enzymatic systems and PC membranes: How do the membrane interactions between the components of an enzymatic system impact enzymatic reactions? For example, the encapsulation of nucleic acid templates for an transcription reaction can be optimized using positively charged amphiphiles, but how does this interaction impact the availability/function of the templates?
  • Supply of encapsulated enzymatic machinery with substrates: What are the possible methods/processes to achieve sustained substrate delivery to the encapsulated enzymatic systems?

Contact: Pierre-Alain Monnard

Project II Bioreactors High through put detection and separation of reactors with active species

This project is conducted in collaboration with SDU Sønderborg that will produce the microfluidic chips. More will follow.

Contact: Pierre-Alain Monnard

Project III Multiple compartment biomimetic bioreactors

Cells are composed of a series of micro- and nanocompartments whose interactions and interconnections permit cell functions. In more simple design (two concentric compartments, basically bilamellar liposomes), we want to explore the possibility of concerted reactions.

  • Preparation of bilamellar liposomes
  • Preparation of bilamellar liposomes with PAHs embedded in the internal lamellae
  • Study of the pH variations in both internal aqueous compartments

Contact: Pierre-Alain Monnard